84-1090-28　Trypsin 1,2,3 (PRSS1,2,3) 100ug　T8669-64

特徴

• Applications:Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Trypsin 1/2/3.
• The detection limit for rhTrypsin 1/2/3 is approximately 1ng/lane under non-reducing and reducing conditions.
• Immunoprecipitation: Used to immunoprecipitate rhTrypsin 1/2/3 from conditioned media of transfected NS0 cells.
• Neutralization: Used to inhibit/neutralize the enzymatic activity of rhTrypsin 2.
• The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes.
• The enzyme was then assayed with a fluorogenic peptide substrate.
• 50% of the proteolytic activity was inhibited by the antibody at approximately 0.3ug/ml (IC50) under conditions in which the enzyme was present at 0.02ug/ml and the substrate concentration was 10uM.
• Considering the molecular masses of the enzyme (26 kD) and the antibody (150 kD), IC50 was achieved at approximately 3:1 molar ratio of the antibody to the enzyme.
• The antibody did not inhibit the activity of rhTrypsin 1 and rhTrypsin 3 significantly
• Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Trypsin 1/2/3.
• The detection limit for rhTrypsin 1/2/3 is approximately 0.8ng/well.
• Optimal dilutions should be determined by each laboratory for each application.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile PBS and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.