Trefoil factor 2 (TFF2), also known as spasmolytic polypeptide (Sp) is a 20kD secreted trefoil factor family protein.
It is a protease-resistant glycoprotein primarily produced by mucous-secreting cells of the stomach and duodenum and is upregulated in response to inflammation.
TFF2 interacts with mucin and other TFF2-binding proteins to protect and repair mucosal epithelium.
TFF2 is also expressed by macrophages and lymphocytes and may have a role in modulating immune system responses.
The 106 residue mature human TFF2 contains two trefoil domains (aa # 36-72 and # 80-121) and one N-linked glycosylation site.
Human TFF2 shares 71% and 87% aa sequence identity with canine and mouse TFF2, respectively.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (direct): 0.5-1ug/ml.
The detection limit for rhTFF2 is ~0.5ng/ml
Western Blot: 0.1-0.2ug/ml.
The detection limit for rhTFF2 is ~5ng/ml under non-reducing and reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human TFF2 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 0.5ml sterile ddH2O or sterile 40-50% glycerol.
Specificity：Recognizes human TFF2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human TFF2 (aa24-129) UniProt # Q03403
