TFPI-2 (tissue factor pathway inhibitor-2; also placental protein 5/PP5) is a secreted, presumably 27-34kD glycoprotein member of the Kunitz domain-containing proteinase inhibitor family.
In mouse, it is principally produced by endothelium, hepatocytes and synctiotrophoblasts, and serves as an inhibitor of multiple serine proteinases.
Mature mouse TFPI-2 is 208aa in length.
It contains three Kunitz inhibitor domains, the most N-terminal of which (aa36-86) is crucial to inhibitor activity.
Over aa23-211, mouse TFPI-2 shares 86% and 58% aa sequence identity with rat and human TFPI-2, respectively.
Applications:Suitable for use in Western Blot, Immunoprecipitation and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml.
Detection limit for rmTFPI-2 is ~20ng/lane and 2ng/lane under non-reducing and reducing conditions
ELISA (direct): 0.5-1ug/ml.
Detection limit for rmTFPI-2 is ~0.1ng/well.
Immunoprecipitation: 25ug/ml Immunoprecipitates rmTFPI-2 from conditioned media of transfected NS0 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse TFPI-2.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu23-Lys211 from mouse TFPI-2 expressed in NS0 cells.
