Thrombomodulin (TM; also fetomodulin and CD141) is a 110-120kD, endothelial cell-specific type I transmembrane glycoprotein.
It binds thrombin, rendering it unavailable for clotting, and activates protein C, which also serves as an anticoagulant.
Mouse Thrombomodulin/TM is 577 amino acids (aa) in length.
It contains a 510aa extracellular domain (ECD) (aa17-517) that shows one C-type lectin domain (aa31-167) and six EGF-like regions (aa240-480).
Thrombin binds to aa403-480.
One isoform may exist that shows a deletion of the C-terminal 86 amino acids (aa492-577).
Over aa17-517, mouse TM is 66%, 85% and 67% aa identical to human, rat and canine TM, respectively.
Applications:Suitable for use in Flow Cytometry and Western Blot.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 50ug/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume ≤200ul.
Tested on bEnd.3 cells
Western Blot: 0.1-0.2ug/ml; The detection limit is ~0.5ng/lane and 25ng/lane under non-reducing and reducing conditions, respectively.
Shows less than 1% crossreactivity with recombinant human Thrombomodulin
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after reciept at -20°C.
Reconstitute with sterile TBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3 (20mM Tris, 150mM sodium chloride) 0.1% BSA.
Specificity：Recognizes mouse Thrombomodulin, extracellular domain at ~110kD. Species Sequence Homology: rat; 85%, canine; 67%, human; 66%
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa17-517 from mouse Thrombomodulin extracellular domain expressed in NS0 cells (P15306).
