Synapsin I is an 85 kD neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release.
It is concentrated in presynaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton.
Phosphorylation at various sites reduces synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release.
Rat synapsin I is 704 amino acids (aa) in length and contains five domains, named A-E/F.
Domains A-D lie between aa 1-655, while E and F are alternate splice forms.
Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner.
Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region.
Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation.
Phosphorylation of S603 and 566 in domain D, and S9 in domain A, reduces the affinity of synapsin I for actin and secretory vesicles.
Applications:Western Blot: 1:1000Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Specific for the ~78kD Synapsin I doublet protein phosphorylated at S9 in Western Blots of rat hippocampal lysates. The antibody also weakly labels the ~55kD Synapsin II, which has a similar phosphorylation site to that of S9 on Synapsin I.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt Xe
Immunogen：Phosphopeptide corresponding to amino acid residues surrounding the phospho-S9 of Synapsin I
