84-1090-15　Synapsin I 50ul　S9104-01D

特徴

• Synapsin I is an 85 kD neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release.
• It is concentrated in presynaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton.
• Phosphorylation at various sites reduces synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release.
• Rat synapsin I is 704 amino acids (aa) in length and contains five domains, named A-E/F.
• Domains A-D lie between aa 1-655, while E and F are alternate splice forms.
• Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner.
• Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region.
• Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation.
• Phosphorylation of Ser603 and 566 in domain D, and Ser9 in domain A, reduces the affinity of synapsin I for actin and secretory vesicles.
• Applications:Western Blot: 1:1000 Optimal dilutions should be determined by each laboratory for each application.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.