84-1090-13　Sulfamidase (N-Sulfoglucosamine Sulfohydrolase, SGSH) 100ug　S8015-18

特徴

• Also known as N-sulfoglucosamine sulfohydrolase and heparan N-sulfatase, Sulfamidase/SGSH is an important member of the sulfatase family involved in the degradation of heparan sulfate (1).
• The SGSH deficiency results in mucopolysaccharidosis type IIIA (MPS IIIA, Sanfilippo A syndrome), an autosomal recessive lysosomal storage disease characterized by neurological dysfunction but relatively mild somatic manifestations (2).
• The deduced amino acid sequence of mouse SGSH consists of a signal peptide (residues 1‑22) and a mature chain (residues 23‑502) (1).
• rmSGSH corresponds to the mature chain and has sulfatase activity.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.2ug/ml Detects mouse SGSH.
• The detection limit is ~5ng/lane under non-reducing and reducing conditions.
• Immunoprecipitation: 25ug/ml Immunoprecipitates rmSGSH from conditioned media of transfected NS0 cells.
• Direct ELISA: 0.5-1.0ug/ml Detects mouse SGSH.
• The detection limit for rmSGSH is ~0.3ng/well.
• Optimal dilution determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.