SCGF, also named CLEC11a, is a 47 kD, 328 amino acid, secreted glycoprotein member of the C-type lectin superfamily.
Produced by CD33+ stem cells and resting chondrocytes, SCGF acts in combination with other factors to promote erythroid, myeloid and endothelial cell production.
Over the region used as immunogen, mouse SCGF is 92% and 83% aa identical to the corresponding rat and human protein sequences, respectively.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse SCGF.
The detection limit for rmSCGF is approximately 1 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse SCGF.
The detection limit for rmSCGF is approximately 0.1 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by mouse SCGF affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Selected for its ability to recognize mouse SCGF in direct ELISAs and Western Blots. In these formats, this antibody shows less than 2% crossreactivity with rhSCGF.
