Stabilin-1, also known as FEEL-1 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor 1) and CLEVER-1 (common lymphatic endothelial and vascular endothelial receptor 1), was originally identified as the MS-1 antigen.
It is a type I transmembrane multi-domain protein with a large extracellular domain that contains 7 fasciclin, multiple EGF-like and laminin type EGF-like, and a link domain.
The short cytoplasmic tail interacts with CGAs which are cargo adaptors that link cargo into the clathrin-coated pits.
Stabilin-1 is expressed by tissue macrophages and sinusoidal endothelial cells, which are scavenging cells.
Stabilin-1 is involved in intracellular trafficking pathways and binds multiple ligands including acetylated LDL, SPARC, AGE, and SI-CLP.
By alternative splicing, a soluble Stabilin-1 containing a 58 residue substitution between amino acid residues 746 and 803, followed by truncation, has been identified.
Within the region used as immunogen, human Stabilin-1 shares 83% and 84% amino acid sequence homology with mouse and canine Stabilin-1, respectively.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Stabilin-1.
The detection limit for rhStabilin-1 is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively.
In this format, this antibody shows less than 1% cross-reactivity with rhStabilin-2.
Optimal dilutions should be determined by each laboratory for each application.
Reconstitution:Reconstitute with sterile Tris-buffered saline pH 7.3 (20 mM Trizma base, 150 mM NaCl) containing 0.1% BSA.
If 1 mL of buffer is used, the antibody concentration will be 50ug/ml.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug BSA per 1ug of antibody.
Specificity：Selected for use as a detection antibody in human Stabilin-1 Western Blots.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant protein corresponding to Gly2208-Val2305 from human Stabilin-1.
