Src kinase-associated phosphoprotein of 55 kD (SKAP55) is an adaptor protein expressed exclusively in mononuclear cells of hematopoietic lineage, with preferential expression in T lymphocytes and monocytes.
Also known as SCAP1, SKAP55 regulates integrin-mediated adhesion and promotes synapse formation between T cells and antigen-presenting cells.
SKAP55 is expressed as a constitutively tyrosine-phosphorylated protein that interacts with the SH2 domain of Fyn in resting cells.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol, 0.02% SODIUM AZIDE.
Specificity：Recognizes human SKAP55.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived full-length recombinant human SKAP55 (aa1-358) UniProt # Q86WV1
