SOX15 (also known as SOX20) is a nuclear, 25 kD transcriptional repressor that belongs to group G of the SOX gene family.
Human SOX15 is 233 amino acids (aa) in length and contains a 63 aa DNA-binding HMG-box (amino acids # 48-110).
Although the C-terminus (aa # 126-233) does not bind DNA, it is required for SOX15 repressive activity.
It may accomplish this by interacting with β-catenin.
SOX15 is expressed in myoblasts and regulates skeletal muscle regeneration and differentiation.
Human SOX15 shares 75% aa sequence identity with mouse SOX15.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human SOX15.
The detection limit for rhSOX15 is approximately 20 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively.
In this format, this antibody shows less than 1% cross-reactivity with rhSOX1, -2, -5, -6, -7, -10, -12, -14, -15, -17, and rhSOX21.
Immunocytochemistry: This antibody can be used at a concentration of 10ug/ml to detect SOX15 in NTera2 teratocarcinoma cells.
Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature.
After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine RedTM-coupled anti-sheep IgG at room temperature in the dark for one hour.
Between each step, cells were washed with PBS containing 0.1% BSA.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human SOX15.
The detection limit for rhSOX15 is approximately 0.3 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human SOX15 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Selected for its ability to recognize human SOX15 in the applications listed.
