SMAGP (small transmembrane and glycosylated protein) is a 25 kD, type III transmembrane glycoprotein that participates in epithelial cell polarity.
Rat SMAGP is 97 amino acids (aa) in length and contains no signal sequence.
The N-terminal 34 amino acids constitute the extracellular domain and show O-linked glycosylation.
The C-terminal 42 amino acids (# 56-97) make up the cytoplasmic region.
SMAGP is likely involved in cell adhesion and communication.
Relocation of SMAGP from the membrane to the cytosol is associated with tissue disorganization and metastatic transformation.
Over the extracellular and cytoplasmic domains, rat SMAGP shares 96% and 66% aa sequence identity with mouse and human SMAGP, respectively.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.
Specificity：Recognizes rat SMAGP.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：E. coli-derived recombinant rat SMAGP (aa1-34 and aa56-97) Accession # Q7TPFI
