Serpin A6 (also known as corticosteroid-binding globulin/CBG and Transcortin) is a 50-60kD, secreted glycoprotein member of the serpin family of protease inhibitors.
It is produced by hepatocytes and transports >90% of circulating cortisol.
Although Serpin A6 is a serpin family member, it shows no protease inhibition.
Mature mouse Serpin A6 is 375aa in length.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Direct ELISA.
Detection limit for recombinant mouse Serpin 6 is ~0.5ng/ml under non-reducing and reducing conditions
Immunohistochemistry: 5-15ug/ml detects mouse Serpin 6 in cells and tissues
Direct ELISA: 0.5-1ug/ml detects mouse Serpin 6.
Detection limit for recombinant mouse Serpin 6 is ~0.5ng/wellOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Serpin A6. Shows ~25% crossreactivty with recombinant rat Serpin A6 and <1% crossreactivity with recombinant human Serpin A1 and recombinant human Serpin A5 when tested in Direct ELISA.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to Val23-Ala397 from mouse Serpin A6, expressed in NSO cells (Accession # Q06770). Species Sequence Homology: rat; 78% and human; 57%.
