84-1089-93 RGMb (Repulsive Guidance Molecule B) 100ug R1994-88
特徴
- Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse and human RGM-B.
- The detection limit for rmRGM-B and rhRGM-B is approximately 1 ng/lane under non-reducing and reducing conditions.
- Immunohistochemistry: This antibody will detect mouse RGM-B in cells and tissues.
- The working dilution is 2-5ug/ml
- Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse and human RGM-B.
- The detection limit for rmRGM-B and rhRGM-B is approximately 0.5 ng/well.
- Optimal dilutions should be determined by each laboratory for each application.
- Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
- Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
- Reconstituted product is stable for 12 months at -20°C.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Further dilutions can be made in assay buffer.
仕様
- Size：100ug
- Host：sheep
- Source Antibody：mouse
- Grade：Affinity Purified
- Purity：Purified by immunoaffinity chromatography.
- Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Specificity：Recognizes mouse RGMb. Less than 5% crossreactivity was observed with recombinant mouse RGMa and RGMc.
- Isotype：IgG
- Calc Applications Abbrev：E IHC WB
- Calc Crossreactivity：Hu Mo
- Immunogen：Recombinant protein corresponding to Gly49-Ser414 from mouse RGMb, expressed in NS0 cells.
- Shelf Life：1year
- Swiss Prot Number：Q7TQ33
- EU Commodity Code：30021010
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