Mouse Renin is a secreted, 42-47kD glycosylated member of the peptidase A1 family.
It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I.
In mouse, there are two genes that code for Renin.
One is in the submandibular gland and the other is in the kidney.
The two mature Renin molecules are 95% amino acid (aa) identical.
Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22-402).
In the kidney, pro-Renin is proteolytically cleaved after Thr71 to generate a mature enzyme.
Mouse pro-Renin shares 70% and 85% aa sequence identity with human and rat pro-Renin, respectively.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml Recombinant mouse ReninImmunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant mouse ReninImmunohistochemistry: 5-15ug/ml using perfusion fixed frozen sections of mouse kidneyELISA (Capture): 0.2-0.8ug/ml using R1460-40 AntibodyOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Renin-1. Species Crossreactivity: rrRenin-1; ~5%, rhRenin-1, <0.2%
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu22-Arg402 from mouse Renin-1, expressed in NS0 cells.
