Regenerating islet-derived protein III alpha (Reg IIIa), also named Pancreatitis Associated-Protein 2 (PAP-2), is a secreted, 17kD member of the Reg family.
These C-type lectin superfamily proteins are involved in various biological functions, including liver and pancreatic regeneration and proliferation.
Reg IIIa is predominately expressed in intestine and exocrine pancreas.
It is also induced in primary liver tumors but is not expressed in normal liver.
Mature mouse Reg IIIa contains 149aa residues and shares less than 60% aa sequence identity with other Reg III proteins.
The protein sequence of mouse Reg IIIa is 87% and 61% identical to that of rat and human Reg IIIa, respectively.
Applications:Suitable for use in Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Reg IIIa. Species Crossreactivity: rat. Crossreactivity: <1% with recombinant human Reg Ib and recombinant mouse Reg I.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to Glu27-Gln175 of mouse Reg IIIa expressed in E. coli. Species Sequence Homology: rat (87%) and human (61%).
