84-1089-88　Reelin 100ug　R1362

特徴

• Reelin is a 400kD, extracellular matrix glycoprotein secreted by several neurons.
• It is a serine protease that degrades fibronectin and laminin.
• Reelin also binds lipoprotein receptor superfamily members APOER2 and VLDLR which transduce signals important for neuronal positioning during brain development and synaptic plasticity in the adult brain.
• In vivo, Reelin undergoes proteolytic processing at two sites, generating three Reelin fragments.
• The N-terminal domain binds integrin alpha3/beta1, leading to a disruption of neuronal glial cell interactions and inhibition of neuronal migration.
• Mouse Reelin shares 94% and 96% amino acid identity with human and rat Reelin, respectively in the region immunized.
• Applications:Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: 5-15ug/ml stains immersion fixed frozen sections of mouse embryo
• Western Blot: 0.1ug/ml stains recombinant mouse reelin
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.