Protein S is a 69kD, secreted glycoprotein that belongs to a general group of vitamin K-dependent proteins.
It is synthesized principally by hepatocytes, circulates both free (40%) and bound to C4bBP (60%), interacts with protein C to suppress coagulation, and binds TAM family RTKs during immune regulation.
Mature human Protein S is 634 amino acids in length.
It contains one Gla domain (aa42-87), four EGF-like repeats (aa117-283) and two laminin G-like domains (aa299-666).
Over aa42-670, human Protein S shares 83%, 80%, and 79% aa sequence identity with porcine, mouse and canine Protein S, respectively.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml.
The detection limit is ~5ng/lane under non-reducing and reducing conditions
ELISA (dierct): 0.5-1ug/ml.
The detection limit is ~0.2ng/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, PBS.
Aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Protein S.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：NS0-derived recombinant human Protein S (aa42-670) UniProt # P07225
