PKC β1 is a 671 aa, 77kD member of the PKC subfamily of Ser/Thr family of protein kinases.
PKC β1 is a Ca2+ -activated, phospholipid-dependent enzyme responsible for the phosphorylation of multiple proteins.
Human PKC β2 is an alternatively spliced isoform with a 52 aa substitution of the 50 C-terminal residues.
In monkey, five alternativly spliced forms have been reported.
Over the region used as immunogen, human and rat PKC β1 are 100% identical.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect PKC β1.
The detection limit for rhPKC β1 is approximately 0.5 ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: This antibody has been used at a concentration of 10ug/ml to detect PKC β1 in neural progenitor cells.
Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature.
After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour.
Between each step, cells were washed with PBS containing 0.1% BSA.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human PKC β1.
The detection limit for rhPKC β1 is approximately 0.3 ng/well.
In this format, this antibody shows less than 1% cross-reactivity with rhPKCδ.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human PKCb1 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
