gondii Profilin is a 163 amino acid, 18kD profilin-like protein that generates a potent interleukin-12 (IL-12) response in murine dendritic cells through binding to Toll-like receptor 11 (TLR11).
TLR11 does not appear to be expressed in human.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml with the appropriate secondary reagents detects T.
gondii Profilin.
The detection limit for T.
gondii Profilin is ~1ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS
Specificity：Recognize T. gondii Profilin in direct ELISAs and Western Blots.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to Ser2-Tyr163 from T. gondii Profilin expressed in E. coli.
