Polo-like kinases are critical regulators of cell cycle progression, cytokinesis, mitosis, and the genotoxic stress response.
Four distinct members have been identified to date in mammalian cells: PLK-1, PLK-2, PLK-3, and PLK-4.
PLK-3 (polo-like kinase 3) plays an important role in cell cycle regulation by modulating microtubule dynamics and centrosomal function.
Although protein levels are unchanged throughout the cell cycle, kinase activity peaks during late S and G2 phases.
Many key cell cycle regulators are phosphorylated by PLK-3 including p53 on Ser20, Cdc25C on Ser216, and the major subunit of DNA polymerase delta, p125 on Ser60.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, 0.02% sodium azide or sterile 40-50% glycerol.
Specificity：Recognizes human PLK-3.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human PLK-3 (aa320-520) GenBank Accession #BC013899
