Plexin D1 is a type I transmembrane protein that is the prototype of the Plexin D subfamily in the Plexin family.
It is expressed on multiple tissues including fetal neuronal and endothelial cells.
Plexin D1 expression is also induced on tumor vasculature and tumor cells.
The 1879 amino acid (aa) mature human Plexin D1 contains a large (1225 aa) extracellular domain with a 500 aa sema domain and three short MRS (Met-related sequences) cysteine-rich motifs.
By alternative splicing, multiple Plexin-D1 isoforms may exist.
Plexin D1 binds Sema4A to suppress angiogenesis.
It also binds Sema3E directly to mediate repulsive vascular vessal guidance.
In mouse, this interaction has been shown to be independent of Neuropilin and Sema3A.
Over the extracellular region used as immunogen, human Plexin D1 shares 89% and 91% aa identity with the mouse and canine protein, respectively.
Applications:Suitable for use in Flow Cytometry, Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 25ug/ml.
Add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul
Western Blot: 0.1-0.2ug/ml.
The detection limit is ~5ng/lane under non-reducing and reducing conditions
Direct ELISA: 0.5-1ug/ml.
The detection limit is ~0.2ng/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile 40-50% glycerol, PBS.
Reconstituted product is stable for 12 months at -20ºC.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
Specificity：Recognizes human Plexin D1. Species sequence homology: mouse 89% and canine 91%.
