Plexin A3 (also known as SEX) is a 220 kD member of the Plexin A subfamily of semaphorin receptors.
Mature mouse Plexin A3 is predicted to be an 1853 amino acid (aa) type I transmembrane glycoprotein that contains a 1202 aa extracellular domain (ECD) and a 629 aa cytoplasmic region.
The ECD contains one semaphorin domain (aa # 34-473), three plexin-semaphorin-integrin (PSI) domains (aa # 492-830), and four lg-like plexin-transcription factor (IPT) repeats (aa # 841-1209).
Plexin A3 interacts with neuropilins 1 and 2, and binds class 3 semaphorins.
Over aa # 35-150, mouse Plexin A3 is 99% and 97% aa identical to rat and human Plexin A3, respectively.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Plexin A3.
The detection limit for rmPlexin A3 is approximately 2 ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: This antibody has been used at a concentration of 10ug/ml to detect Plexin A3 in cryosections of E13 mouse embryos.
Tissue was fixed overnight in 4%PFA+14%picric acid in PBS at 4° C.
Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 minutes at room temperature.
After blocking, sections were incubated with primary antibody for 3 hours at room temperature followed by NorthernLightsTM 557 donkey anti-goat lgG at room temperature for 1 hour.
Between each step, sections were washed with 0.1% BSA in PBS.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Plexin A3.
The detection limit for rmPlexin A3 is approximately 1.0 ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by mouse Plexin A3 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Selected for its ability to recognize mouse Plexin A3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5% crossreactivity with rmPlexin A1, rmPlexin A2, rhPlexin B1, rhPlexin C1, and rhPlexin D1.
