PGCP (plasma glutamate carboxypeptidase) is a secreted member of the M28 family of metallopeptidases.
Mouse PGCP is 470 amino acids (aa) in length.
It apparently contains one annexin-like region (aa 19-68) plus a peptidase M28 catalytic domain (aa 281-443).
It appears to be synthesized by the liver.
Based on human, mouse PGCP will show a 97 aa substitution for the C-terminal 26 amino acids.
Over aa's 19-470, mouse PGCP shares 89% and 94% aa identity with human and rat PGCP, respectively.
Applications:Suitable for use in Western Blot, Immunoprecipitation and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by mouse Mcpt6 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity：Recognizes mouse PGCP.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Mo
Immunogen：Mouse myeloma cell line NS0-derived recombinant mouse PGCP, Lys19-Ser470.
