PCOLE1 (PCPE-1) is a secreted 55kD enzyme adaptor that enhances the activity of the BMP-1/tolloid family of metalloproteinases (also known as procollagen C-proteinases) on fibrillar procollagens.
PCOLE1 binds to the C-propeptide region and also elsewhere in the procollagen molecule to specifically stimulate BMP-1/tolloid processing of procollagen I, II, and III.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/mlImmunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with Recombinant Mouse PCPE‑1Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse PCOLE1. Shows ~35% crossreactivity with recombinant human PCPE.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln25-Ala468 (predicted) from mouse PCOLE1, expressed in NS0 cells.
