The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7.
Paraoxonase/arylesterase 2 (PON2) is a 354 amino acid, 39 kD protein that is widely expressed in a variety of tissues and may act as a cellular antioxidant, protecting cells from oxidative stress.
PON2 has arylesterase and aryldialkylphosphatase activity (EC 3.1.1.2 and EC 3.1.8.1) and can hydrolyze a number of organophosphate substrates and aromatic carboxylic acid esters.
PON2 is membrane-bound and has several potential glycosylation sites.
Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes.
PON2 is not associated with HDL but can prevent LDL lipid peroxidation and reverse the oxidation of mildly oxidized LDL.
Alternatively spliced transcript variants encoding different isoforms have been described.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile buffer or ddH2O.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 100ul sterile PBS containing 0.02% SODIUM AZIDE.
Specificity：Recognizes endogenous human PON2. Does not cross-react with recombinant human PON1 or PON3 in Western blots.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human Paraoxonase 2 (aa30-354) SwissProt # Q15165
