84-1089-68　Paired Immunoglobulin-Like Type 2 Receptor beta (PILRb) 100ug　P2150-50

特徴

• Paired immunoglobulin-like, type 2 receptor beta (PILR-beta) is a type I transmembrane (TM) glycoprotein belonging to the Ig superfamily.
• It is the activating counterpart to the ITIM-bearing PILR-alpha inhibitory receptor.
• PILR-beta is expressed in a wide variety of tissues including hematopoietic cells.
• Mature human PILR-beta is a 208 amino acid (aa) protein with one V-type Ig-like extracellular domain, a truncated cytoplasmic tail, and positively-charged residues in its TM domain that interacts with ITAM-bearing adaptor molecules.
• Within the V-type Ig-like region in their ECD, human PILR-beta and PILR-alpha share a 92% aa sequence identity.
• The aa sequence of mouse PILR-beta ECD is only 43% identical to that of the human protein.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.2ug/ml.
• The detection limit is ~1ng/lane under non-reducing and reducing conditions.
• In this format, this antibody shows ~10% cross-reactivity with recombinant human PILR-alpha and <5% cross-reactivity with recombinant mouse PILR-beta, PILR-alpha, and PILR-L.
• Direct ELISA: 0.5-1ug/ml.
• The detection limit for is ~0.3ng/well.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.