84-1089-66 NTB-A (SLAMF6, SLAM family member 6, Activating NK receptor, KALI, KALIb, Ly108, MGC104953, NK-T-B-antigen, NTBA, SF2000, UNQ6123/PRO20080) 100ug N6000-35E
84-1089-66 NTB-A (SLAMF6, SLAM family member 6, Activating NK receptor, KALI, KALIb, Ly108, MGC104953, NK-T-B-antigen, NTBA, SF2000, UNQ6123/PRO20080) 100ug N6000-35E
Mouse NTB-A also known as Ly108, SLAM6 and SF2000, is a 60kD type I transmembrane glycoprotein that belongs to the SLAM/CD2 family of immune receptors.
NTB-A is expressed in non-terminally differentiated T- and B-cells, NK cells, macrophages and neutrophils.
NTB-A is a 351 aa peptide containing a 209 aa extracellular domain and an 89 aa cytoplasmic tail.
There is at least one mouse NTB-A splice variant that contains a 4-aa substitution for the 24 residues in the C-terminal cytoplasmic region.
Over the region used as immunogen, the aa sequence of mouse NTB-A is 72% and 48% identical to the rat and human proteins, respectively.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse NTB-A.
The detection limit for rmNTB-A is approximately 5 ng/lane under non-reducing and reducing conditions
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse NTB-A.
The detection limit for rmNTB-A is approximately 0.3 ng/well
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:sheep
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by mouse NTB-A affinity chromatography.
Form:Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity:Selected for its ability to recognize mouse NTB-A in direct ELISAs and Western Blots.