84-1089-63　NMDA 2a Receptor (NMDA R2a, N-methyl-D-aspartate) 50ul　N2905-05B

特徴

• NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels.
• The functional NMDA receptor (NMDAR) is a 650-850 kD heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits.
• NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity.
• In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members.
• Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell.
• There are four genes that code for NR2 subunits (NR2A-D).
• The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers.
• The 2A form generates the highest conductance NMDAR.
• Rat NR2A is a 180 kD, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region.
• The molecule is described as 4-TM.
• However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular.
• The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitute the glutamate binding site.
• Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.
• Applications:Western Blot: 1:1000 Optimal dilutions should be determined by each laboratory for each application.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.