NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels.
The functional NMDA receptor is a heteromultimer of at least two NR1 (NMDA receptor-1) subunits and two NR2 subunits.
Regulation of the NMDA complex by external factors such as zinc, PKC and polyamines is mediated by NR1 subunits.
Upon glycine binding to NR1, and glutamate binding to NR2, the NMDA channel is opened, allowing calcium and sodium influx into the cell.
NR1 is a 120 kD, 938 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 541 aa extracellular domain and a 105 aa cytoplasmic region.
The molecule is described as 4-TM.
However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular.
Differential splicing of three exons generates up to nine isoforms of NR1.
These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2).
Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2').
Eight are integral membrane proteins.
At the N-terminus, four isoforms show a 21 aa insertion between aa 190-191, and four do not.
This is compounded by at least four possible variants between aa 864-938 in the cytoplasmic tail that impact potential serine phosphorylation sites.
To date, all are believed to be physiologically active.
There is one potentially "soluble" form that is only 181 aa in length.
Its function is unknown.
Applications:Western Blot: 1:1000 Immunohistochemistry: 1:1000 (frozen sections) Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：25ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mM ammonium bicarbonate.
Specificity：Recognizes mouse and rat ~120kD NR1 subunit of the NMDA Receptor containing the C2 splice variant insert.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Rt
Immunogen：Peptide from the NR1 subunit, C2 Splice Variant Insert of the NMDA Receptor
