Mouse Natriuretic Peptide Receptor-A (NPR-A), also known as NPR-1, ANP-R1 or guanlyl cyclase-A, is a 119 kD member of the guanylyl cyclase family of proteins.
NPR-1 is a 1029 amino acid type I transmembrane glycoprotein that contains a 441 amino acid extracellular domain and a 567 amino acid cytoplasmic region.
The cytoplasmic region shows a tyrosine kinase and guanylyl cyclase catalytic domain.
NPR-1 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B-type n.p.) ANP binding induces bronchodilation and a reduction in blood pressure.
Over the extracellular domain, mouse NPR-1 is 97% and 86% identical to rat and human NPR-1, respectively.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml.
Detection limit is ~10ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml.
Detection limit is ~0.5ng/well
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Natriuretic Peptide Receptor A. Crossreactivity: recombinant human NPR-1 (50%), recombinant human NPR-2 (5%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：NS0-derived recombinant mouse Natriuretic Peptide Receptor A extracellular domain (aa29-469) (UniProt # P18293). Species Sequence Homology: rat (97%), human (86%).
