Mxi-1 (Max interactor 1) is a 26kD member of the bHLH-leucine zipper family, Myc superfamily of transcription factors.
It is a widely expressed transcriptional repressor that heterodimerizes with Max and blocks Max-Myc gene activation.
Human Mxi-1 is 228 amino acids (aa) in length.
It contains a DNA binding motif (aa 69-80) and a bHLH domain (aa 81-120).
Multiple splice variants of Mxi-1 appear to exist.
They may incorporate an alternate start site at Met37, a 92 aa substitution for the first 26 N-terminal amino acids, or a deletion of the C-terminal 59 or 74 amino acids.
Over aa 81-228, human Mxi-1 is 95% aa identical to mouse Mxi-1.
Applications:Suitable for use in Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 10ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human Mxi1 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity：Recognizes human MXI1.
Isotype：IgG
Calc Applications Abbrev：E IC
Calc Crossreactivity：Hu
Immunogen：E. coli-derived, recombinant human Mxi1 (aa81-228) UniProt # P50539
