Beta-2 microglobulin is a ubiquitous, 12kD, secreted, non-glycosylated protein required for cell surface expression and non-covalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins.
Mature rat b2-Microglobulin is a 99aa peptide containing one C1-type lg-like domain (aa 22-116).
In human, b2-Microglobulin is known to dissociate from the MHC complex and circulate as full-length and N-terminal-truncated peptides of 93, 91 and 90 amino acids.
Applications:Suitable for use in Direct ELISA, Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Direct ELISA: 0.5-1.0ug/ml.
The detection limit is ~1ng/well
Western Blot: 0.1-0.2ug/ml.
The detection limit is ~1ng/lane under non-reducing and reducing conditions
Flow Cytometry: 50ug/ml.
10ul labels 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.
Tested on rat splenocytes.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes rat b2-Microglobulin. Species Sequence Homology: mouse; 86%, human; 75%
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Rt
Immunogen：NS0-derived recombinant rat b2-Microglobulin (aa21-116, Accession #PO7151).
