84-1089-51 Methionine Aminopeptidase 1 (MetAP-1) 100ug M3015-98A
特徴
- Applications:Suitable for use in Western Blot, Immunoprecipitation and ELISA.
- Other applications not tested.
- Recommended Dilution:Western Blot: 0.1-0.2ug/ml.
- The detection limit is ~5ng/lane under non-reducing and reducing conditions.
- Shows < 1% crossreactivity with recombinant mouse MetAP-1
- Immunoprecipitation: Immunoprecipitates recombinant human MetAP-1 from conditioned media of transfected NSO cells
- Direct ELISA: 0.5-1ug/ml.
- The detection limit is ~2ng/well
- Optimal dilutions to be determined by the researcher.
- Storage and Stability:Lyophilized powder may be stored at -20°C.
- Stable for 12 months at -20°C.
- Reconstitute with sterile PBS.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Reconstituted product is stable for 12 months at -20°C.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Further dilutions can be made in assay buffer.
仕様
- Size:100ug
- Host:goat
- Source Antibody:human
- Grade:Affinity Purified
- Purity:Purified by immunoaffinity chromatography.
- Form:Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Specificity:Recognizes human Methionine Aminopeptidase 1 (MetAP-1).
- Isotype:IgG
- Calc Applications Abbrev:E IP WB
- Calc Crossreactivity:Hu
- Immunogen:Sf21-derived recombinant human Methionine Aminopeptidase 1 (aa52-386)
- Shelf Life:1year
- Swiss Prot Number:P53582
- EU Commodity Code:30021010
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