Hepatocyte growth factor receptor (HGF R), also known as c-MET, is a receptor tyrosine kinase that consists of an extracellular alpha chain and a longer beta chain.
The beta chain contains the transmembrane helix and the cytoplasmic portion.
Ligand-stimulated phosphorylation of tyrosine 1234 and tyrosine 1235 in HGF R's beta chain results in stimulation of intrinsic kinase activity.
Applications:Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 2.5ug/1x10e6 cells using MCF-7 human breast cancer cell line treated with pervanadate, fixed with paraformaldehyde and permealized with methanol
Immunohistochemistry: 5-15ug/ml using immersion fixed, frozen section of mouse embryo
Western Blot: 0.5ug/ml using pervanadate-treated MDA-MB-468 human breast cancer cell lineOptimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile PBS.
Aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 250ul sterile PBS.
Specificity：Recognizes human and mouse HGF R/c-MET when phosphorylated at Y1234/Y1235
Isotype：IgG
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Phosphopeptide containing human HGF R/c-MET Y1234/1235 sites
