MCPH1 (microcephalin 1), originally identified as an inhibitor of hTERT expression, has been implicated in DNA damage response.
MCPH1 contains one N-terminal and two C-terminal BRCT domains.
BRCT domains are found predominantly in cell cycle proteins responsive to DNA damage.
MCPH1 forms irradiation-induced nuclear foci (IRIF) that colocalize with NBS1, 53BP1, MDC1, and ATM and are abolished with MCPH1 specific siRNA.
MCPH1 also regulates the ATR pathway.
It colocalizes with ATR and RPA and is required for the phosphorylation of RPA and Rad17.
Defects in MCPH1 are the cause of primary microcephaly 1, an autosomal recessive neurodevelopmental disorder.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml using HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, or Neuro-2A mouse neuroblastoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a powder lyophilized from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human MCPH1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Met1-Lys250 from human MCPH1, expressed in E. coli.
