Chymase (also mast cell protease I) is a secreted, 30kD glycoprotein member of the granzyme subfamily, peptidase S1 family of enzymes.
It is secreted by mast cells and vascular smooth muscle cells, and converts angiotensin I into angiotensin II.
This promotes fibroblast proliferation and secretion of ECM.
Mature human chymase is 226aa in length.
It contains one peptidase S1 domain (aa22-245).
There is one potential alternate splice form.
It shows a deletion of the 99 C-terminal amino acids (aa49-247).
Applications:Suitable for use in Western Blot, Immunoprecipitation and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using lysate of human small intestine tissueImmunoprecipitation: 25ug/ml using rhChymase from conditioned media of transfected NS0 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Chymase. Crossreactivity: ~10% with MCPT1 and <1% with rhGranzyme-B, rhGranzyme-H, rmMCPT6 and rmMCPT7.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly20-Asn247 from human Chymase expressed in NS0 cells. Swiss/UniProt Accession: P23946.
