Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Direct ELISA: 0.5-1ug/ml.
The detection limit is ~0.3ng/well
Western Blot: 0.1ug/ml.
The detection limit is ~5ng/lane and 1ng/lane under non-reducing and reducing conditions, respectively.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Mannan Binding Lectin 1. Shows ~10% crossreactivity with recombinant human Mannan Binding Lectin and recombinant mouse Mannan Binding Lectin 2 in Western Blot under reducing conditions.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser18-Ala239 from mouse Mannan Binding Lectin 1, expressed in NS0 cells.
