MAD-1L1 (mitotic arrest deficient 1-like 1) is a member of the spindle-assembly checkpoint (SAC) which prevents anaphase from occurring until all chromosomes are properly aligned at the metaphase plate.
Upon checkpoint activation, MAD-1L1 recruits MAD2 to unattached kinetochores, promoting the binding of MAD2 to CDC20, the activator for the anaphase-promoting complex.
MAD-1L1 has been implicated to have tumor-suppressing functions, but in small-cell lung cancer cell lines, the MAD-1L1 gene is the most frequent copy number gain, suggesting a more complex role.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS
Specificity：Recognizes human MAD-1L1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human MAD-1L1 (aa1-350) GenBank Accession #NM_003550
