DC-LAMP (dendritic cell lysosome-associated membrane protein: also known as CD208 and LAMP3) is a 50-70kD member of the LAMP family of proteins.
Mature human DC-LAMP is a 389 amino acid type I transmembrane protein.
It has a 254aa lumenal N-terminus and a short 14aa cytoplasmic tail.
The molecule is found in type II pneumocytes, interdigitating DC, and various tumors.
DC-LAMP serves as a marker of human mature DC, and it may play a role in normal lysosome and endosome function.
Over the region used as immunogen, human DC-LAMP shares 72% and 55% aa sequence identity with canine and mouse DC-LAMP, respectively.
Applications:Suitable for use in ELISA, Western Blot and Flow Cytometry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml Detection limit is ~0.5 ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1.0ug/ml Detection limit is ~0.1 ng/well.
Flow Cytometry: 25ug/ml.
For monocyte-derived mature dendritic cells, use 10ul of diluted solution for 1-2.5x10e5 cells in total volume ≤200ul
Optimal dilutions to be determined by researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human LAMP3. In ELISA, displays less than 1% cross-reactivity with rhLAMP.
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa21-381 of human DC-LAMP lumenal domain expressed in NS0 cells (EAW78337). Species sequence homology: canine 72%, mouse 55%.
