Lipocalin 2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1-3).
It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (KD = 0.41nM) (4).
The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA).
It also binds to a ferric DHBA complex with much less KD values (7.9nM) (4).
Secretion of Lipocalin 2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection.
As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5).
Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6).
In the kidney, Lipocalin 2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:ELISA (Capture): 0.2-0.8ug/mlWestern Blot: 1ug/ml detects Lipocalin 2 in rat ovary tissue and mouse lung tissue
Immunoprecipitation: 25ug/ml immunoprecipitates Lipocalin 2 from conditioned cell medium spiked with recombinant rat Lipocalin 2.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes rat Lipocalin 2 at ~25kD. Shows <0.4% crossreactivity in sandwich ELISAs with recombinant mouse Lipocalin 2 and <0.2% crossreactivity with recombinant human Lipocalin 1 and Lipocalin 2. Species Crossreactivity (Western Blot): mouse
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to Gln21-Asn198 from rat Lipocalin 2 expressed in NS0 cells
