Versican (versatile proteoglycan; also known as PG-M) is a 500-600kD, secreted member of the Aggrecan/Versican proteoglycan family.
It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices.
Human Versican is 3396 amino acids (aa) in length.
It contains an N-terminal hyaluronan-binding region (G1; aa21-346), two large, alternately spliced GAG attachment domains (GAGalpha; aa348-1335; GAGbeta aa1336-3088) and a C-terminal selectin-like region (G3; aa3089-3396).
Over aa1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.
Applications:Suitable for use in Direct ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml detects Versican in recombinant human Versican isoform V0.
Immunocytochemistry: 5-15ug/ml detects Versican in immersion fixed HeLa cells.
Immunohistochemistry: 5-15ug/ml detects Versican in immersion fixed frozen sections of mouse embroyo.
Optimal dilutions should be determined by each laboratory.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Large Proteoglycan. Species Crossreactivity: mouse (IHC)
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to Gly1344-Asp1554 from human Versican isoform V0, expressed in E. coli. Species Sequence Homology: canine; 80%, mouse; 60%
