Laminins are heterotrimeric glycoproteins and are major components of the basement membrane.
Each laminin is comprised of a single α, β, and γ chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule.
Mature Laminin α4 is a subunit of Laminin 411, Laminin 421, and Laminin 423.
Mature Laminin α4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C-terminal tandem Laminin G-like domains.
The G-like domains contain binding sites for integrin, heparin, and dystroglycan.
Within the region used as immunogen, mouse Laminin α4 shows 91% and 97% aa sequence identity with human and rat Laminin α4, respectively.
Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect recombinant mouse Laminin α4.
The detection limit for rmLaminin α4 is approximately 10 ng/lane under non-reducing and reducing conditions.
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
Specificity：Selected for use as a detection antibody in mouse Laminin a4 Western Blots.
