Laminin subunit Alpha-1 (LAMA1) is a secreted 400kD extracellular matrix glycoprotein that contributes to the formation of basement membrane Laminin isoforms 1 and 3.
It is one of three subunits (alpha, beta, and gamma) that interact via their coiled-coil domains to form the approximately 800kD cruciform, disulfide-linked, Laminin heterotrimer.
The 3058 amino acid (aa) residue mature human alpha-1 chain contains an N-terminal Laminin VI domain (aa18-269), followed by domains V through III containing 17 EGF-like repeats, the coiled-coil domains II and I, and five globular, Laminin G-like domains.
Over aa22-269, human Laminin alpha-1 shares 95% and 91% aa sequence identity with canine and mouse alpha-1 chain, respectively.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Direct ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/mlImmunohistochemistry: 5-15ug/ml using immersion fixed frozen sections of mouse embryo
Flow Cytometry: 10ul labels10e6 cells.
Validated on U2OS human osteosarcoma.
For intracellular staining, cells must first be fixed and permeabilized.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Laminin alpha 1 N-Terminus Domain VI. Less than 1% crossreactivity was observed with recombinant mouse LAMA4.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu22-Met269 from the N-Terminal domain VI of human Laminin alpha 1, expressed in E. coli.
