Kallikrein 15, encoded by the KLK15 gene, is a member of the tissue Kallikrein family of Serine proteases.
It is a secreted protein found in prostate, colon, thyroid tissues, breast milk and seminal plasma.
Whether it has a trypsin-like activity or not remains controversial.
Several splice forms exist.
The 250 aa rhKLK15 has a 10-His tag at the C-terminus.
It predicts a molecular mass of 28kD and migrates as a 39kD band in SDS-PAGE under reducing conditions.
The amino acid sequence of human KLK15 is 99%, 77% and 77% identical to that of chimpanzee, canine and mouse, respectively.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/mlImmunohistochemistry: 5-15ug/mlImmunoprecipitation: 25ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human KLK15 in direct ELISAs and Western Blots. In these formats, this antibody shows <1% crossreactivity with rhKLK2, rhKLK8, and rhKLK11.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp17-Asn255 of human KLK15, expressed in NS0 cells.
