Applications:Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human KLK13.
The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions.
Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells.
The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody.
Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13.
The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes.
The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC.
50% of the proteolytic activity was inhibited by the antibody at approximately 8.6ug/ml (IC50) under conditions in which the enzyme was present at 1ug/ml and the substrate concentration was 100 μM.
Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 molar ratio of the antibody to the enzyme.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human KLK13.
The detection limit for rhKLK13 is approximately 1.0 ng/well
Optimal dilutions should be determined by each laboratory for each application.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by human KLK13 affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity：Selected for its ability to recognize human KLK13 in direct ELISAs and Western Blots. In these formats, this antibody shows ~10% crossreactivity with rhKallikrein 8, and less than 5% crossreactivity with rhKallikrein 11, rhKallikrein 14, and rhKallikrein 15.
