IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that regulate signaling from Toll-like receptor (TLR) and IL-1 receptor family members.
Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4.
All IRAKs are multidomain proteins, containing a conserved N-terminal death domain as well as a central kinase domain that is only active in IRAK1 and IRAK4.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile PBS.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes endogenous human IRAK1 at ~80kD. Does not detect recombinant human IRAK2 or IRAK4; crossreactivity with IRAK-M is not known.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ser693 from human IRAK1, expressed in E. coli.
