Applications:Suitable for use in ELISA, Immunocytochemistry, Neutralization and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: 0.2-0.8ug/ml as capture antibodyImmunocytochemistry: 5-15ug/ml using immersion fixed human PBMCs treated with PMA and calcium ionomycinNeutralization: Measured by its ability to neutralize IL33induced proliferation in the D10.G4.1 mouse helper T cell line.
The Neutralization Dose (ND50) is typically 0.753.0ug/ml in the presence of 1 ng/mL recombinant human IL33 and suboptimal amounts of mouse CD3-epsilon monoclonal antibody
Western Blot: 0.1ug/ml using rhIL-33 as sample
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with sterile PBS.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a powder lyophilized from a from a 0.2um filtered solution in PBS containing trehalose. Reconstitute to 0.2mg/ml with 500ul sterile PBS.
Specificity：Recognizes human IL-33. Less than 0.05% cross-reactivity with recombinant mouse IL-33 is observed in ELISA.
Isotype：IgG
Calc Applications Abbrev：E IC N WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human IL-33 (aa112-270)
