Applications:Suitable for use in Flow Cytometry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/ml.
Detection limit for recombinant canine IL-1beta is ~0.5ng/lane under non-reducing and reducing conditions.
Shows ~40% cross-reactivity with recombinant feline IL-1beta, 10% cross-reactivity with recombinant human IL-beta, rrmIL-1beta, recombinant equine IL-1 beta, recombinant porcine IL-1beta, and 5% cross-reactivity with rmIL-1beta, recombinant rat IL-1beta, and rcrIL-1beta.
Flow Cytometry: Dilute to 50ug/ml.
Use 10ul of dilution to 1-2.5x10e5 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile TBS, 0.1% BSA and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS and BSA. Labeled with Biotin. Reconstitute with sterile TBS (20mM Trizma vase, 150mM NaCl), 0.1% BSA.
