Integrin beta5 is a 100-110kD type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin aV.
Integrin aVb5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium.
The 696 amino acid residue Integrin b5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region.
Applications:Suitable for use in Western Blot, Flow Cytometry, Immuncytochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.2ug/ml.
The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 50ug/ml, 10ul labels 1-2.5x10e5 MG-63 human osteosarcoma cells in a total reaction volume <200ul.
The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome.
Immunocytochemistry: 10ug/ml detects Integrin, beta5 in MG-63 human osteosarcoma cells.
Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT.
After blocking, cells were incubated with diluted I7661-43E overnight at 4° C followed by Rhodamine Red coupled anti-sheep IgG at RT in the dark for one hour.
Between each step, cells were washed with PBS, 0.1% BSA.
Direct ELISA: 0.5-1.0ug/ml.
The detection limit is ~0.2ng/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Integrin beta5. Shows <5% crossreactivity with recombinant human Integrin beta1, beta3, beta4, beta6 and recombinant mouse Integrin beta7. Species Sequence Homology: mouse; 91%, canine; 89%
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Integrin beta5, Gly24-Asn719, at the extracellular domain, CHO cell-derived
